Automated species identification for camera trapping in the Iona Skeleton Coast Trans-Frontier Conservation Area
The Iona Skeleton Coast Trans-Frontier Conservation Area (TFCA), straddling the border between Angola and Namibia, has suffered through decades of civil war and poaching. While this history has been detrimental to the community of large mammals in the TFCA, data collected on the mammal populations are insufficient to enable effective management. Survey methods such as […]
Three-gene PCR and high-resolution melting analysis for differentiating vertebrate species mitochondrial DNA for biodiversity research and complementing forensic surveillance
Reliable molecular identification of vertebrate species from morphologically unidentifiable tissue is critical for the prosecution of illegally-traded wildlife products, conservation-based biodiversity research, and identification of blood-meal hosts of hematophagous invertebrates. However, forensic identification of vertebrate tissue relies on sequencing of the mitochondrial cytochrome oxidase I (COI) ‘barcode’ gene, which remains costly for purposes of screening […]
The extinct, giant giraffid Sivatherium giganteum: skeletal reconstruction and body mass estimation
Sivatherium giganteum is an extinct giraffid from the Plio–Pleistocene boundary of the Himalayan foothills. To date, there has been no rigorous skeletal reconstruction of this unusual mammal. Historical and contemporary accounts anecdotally state that Sivatherium rivalled the African elephant in terms of its body mass, but this statement has never been tested. Here, we present […]
Detection and characterisation of papillomavirus in skin lesions of giraffe and sable antelope in South Africa
Papillomavirus was detected electron microscopically in cutaneous fibropapillomas of a giraffe (Giraffa camelopardalis) and a sable antelope (Hippotragus niger). The virus particles measured 45 nm in diameter. Histopathologically, the lesions showed histopathological features similar to those of equine sarcoid as well as positive immunoperoxidase-staining of tissue sections for papillomavirus antigen. Polymerase chain reaction (PCR) detected […]
Conservation monitoring: estimating mammal densities in woodland habitats
Conservation and ecological monitoring programmes often estimate animal densities over time, but in wooded and forested areas practical techniques are still poorly developed. Here I have examined five simple methods of deriving densities of large and medium-sized mammals using line transects driven through miombo woodland habitat in Africa. These methods calculated area by dividing the […]