Characterization of eight polymorphic loci for Maasai giraffe (Giraffa camelopardalis tippelskirchi) using non-invasive genetic samples

Author(s)Rachel S. Crowhurst, Tom D. Mullins, Benezeth M. Mutayoba and Clinton W. Epps
Year Published2013
JournalConservation Genetics Resources
Page Numbers85–87
Size160.25 KB
Abstract:

We used Illumina sequencing to develop eight novel microsatellite loci for Maasai giraffe (Giraffa camelopardalis tippelskirchi), and screened them using fecal DNA. Genetic diversity was assessed for 40 individuals from the Katavi National Park/Rukwa Game Reserve ecosystem in Tanzania. The number of alleles per locus ranged from 2 to 14 (mean = 6.2) and mean expected heterozygosity was moderate (range 0.025–0.838, mean = 0.527). These markers were successfully tested using degraded DNA and may be useful for future studies of giraffe, especially those that use non-invasive sampling techniques.

Keywords: Giraffa camelopardalis, Microsatellite, Illumina sequencing, Non-invasive sampling

Authors: Rachel S. Crowhurst, Tom D. Mullins, Benezeth M. Mutayoba and Clinton W. Epps

Journal: Conservation Genetics Resources


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