Three-gene PCR and high-resolution melting analysis for differentiating vertebrate species mitochondrial DNA for biodiversity research and complementing forensic surveillance

Author(s)Daniel O. Ouso, Moses Y. Otiende, Maamun M. Jeneby, Joseph W. Oundo, Joel L. Bargul, Scott E. Miller, Lillian Wambua and Jandouwe Villinger
Year Published2020
JournalScientific Reports
Page Numbers1-13
Size1.51 MB
Abstract:

Reliable molecular identification of vertebrate species from morphologically unidentifiable tissue is critical for the prosecution of illegally-traded wildlife products, conservation-based biodiversity research, and identification of blood-meal hosts of hematophagous invertebrates. However, forensic identification of vertebrate tissue relies on sequencing of the mitochondrial cytochrome oxidase I (COI) ‘barcode’ gene, which remains costly for purposes of screening large numbers of unknown samples during routine surveillance. Here, we adapted a rapid, low-cost approach to differentiate 10 domestic and 24 wildlife species that are common in the East African illegal wildlife products trade based on their unique high-resolution melting profiles from COI, cytochrome b, and 16S ribosomal RNA gene PCR products. Using the approach, we identified (i) giraffe among covertly sampled meat from Kenyan butcheries, and (ii) forest elephant mitochondrial sequences among savannah elephant reference samples. This approach is being adopted for high-throughput pre-screening of potential bushmeat samples in East African forensic science pipelines.

Keywords: Forensic science, PCR-HRM, Species identification, Mitochondrial DNA, Bushmeat, Illegal wildlife products trade

Authors: Daniel O. Ouso, Moses Y. Otiende, Maamun M. Jeneby, Joseph W. Oundo, Joel L. Bargul, Scott E. Miller, Lillian Wambua and Jandouwe Villinger

Journal: Scientific Reports


FileAction
2020-Ouso_Three-gene-PCR-and-highresolution-melting-analysis-for-diferentiating-vertebrate-species-mitochondrial-DNA-for-biodiversity-research-and-complementing-forensic-surveillance.pdfDownload 
Terms and Conditions: Any PDF files provided by the GRC are for personal use only and may not be reproduced. The files reflect the holdings of the GRC library and only contain pages relevant to giraffe study, and may not be complete. Users are obliged to follow all copyright restrictions.